Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Usage Physical State: Freeze-dried solidStorage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:None(Warning:Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.) Suggested Working Concentration or Dilution Range:1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
ConjugateHorseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
Images & ReferencesThis product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.
ERBU——提供用于多抗与单抗生产的高性价比佐剂
基本信息:
产品随货附有说明书有详细的注射步骤. 根据要求提供.
所有的GERBU佐剂是细胞和体液免应答长期保护作用强大的增效剂。非常温和,操作简便,只需要混合抗原并注射。所有的组分均是无毒且完全不会引起排斥反应的。需要频繁的刺激以达到高滴度的免疫反应。
活性成分:
GERBU的效果取决于糖肽GMDP的协调(N-Acetyl-glucosaminyl-(β1,4) -N-acetylmuramyl -l-alanyl-d - isoglutamine ),保加利亚乳杆菌细胞壁亚单位,固体可降解的脂质微滤颗粒构成石蜡油,更重要的是生物乳化剂、增效协调和稳定的环境.
使用建议:
混匀抗原溶液: 通常佐剂和抗原溶液的体积比是1:1,也可以做调整。
Withdrawal of the adjuvant: 使用无菌塑料注射器,将需要的量转移到塑料管里,充分混匀佐剂和抗原。不是乳剂制备,只是抗原混合。
稀释: 正常情况下抗原和佐剂的体积比是1:1。然后也可以根据抗原的浓度调整抗原的体积。
稀释液: 水是最好的稀释液,也可以添加一些HEPES、MOPS或者甘氨酸。缓冲液应避免与多价阴离子如磷酸盐或柠檬酸等,会引起的带正电荷颗粒的絮凝物。pH值为5到6之间被证明比中性条件更好。预制好的混合物需要冷冻保存。
应用: 推荐皮下注射。肌肉注射通常会对动物有伤害。例如在鸡的胸肌处进行肌肉注射后可能造成鸡停止下蛋。
保存: 最好保存在4-8°C。在这个温度下,佐剂变得粘稠,颗粒悬浮液是高度稳定的。在使用佐剂前,需要在将其平衡至室温。
*仅供科研和生产使用
GERBU 佐剂 M # 3001
用于单克隆抗体的生产
研究表明Gerbu用于小鼠单克隆抗体的生产,IgG和融合蛋白的产量都有所提高。佐剂M是0.1毫升包装的,也可以为工业客户提供性价比较高的1ml规格。
-大剂量的 Gmdp
-无皂素
Antigen: Alkaline Phosphatase
With kind permission of René Fischer Biochemistry Laboratories ETH Zürich
GERBU ADJUVANT M – There is no alternative for increasing Mab Yields
GERBU 佐剂 P # 3111 用于小型到中等大小动物的多克隆抗体的生产 改进经典的GERBU佐剂 - 含有季胺盐化合物阳离子复合物 |
GERBU ADJUVANT F # 3030 用于大型动物 一种高度浓缩商业用途的佐剂 为了使用最小的量达到最强的效果。精心调整的协同和稳定介质包围的纳米粒子,开发效率,特别是在大型动物。 - 无皂素 - 较少的稳定剂 |